Kallikrein 2 Antibody

Western Blot: Kallikrein 2 Antibody [NBP1-06046] - Staining of HeLa cell lysate (A) + peptide (B) (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Kallikrein 2 Antibody [NBP1-06046] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Kallikrein 2 Antibody [NBP1-06046] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: Kallikrein 2 Antibody [NBP1-06046] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kallikrein 2 Antibody Summary

• Immunogen: Synthetic peptide, C-KHQSLRPDEDSSHD, representing this region of the sequence according to NP_005542.1; NP_001002231.1
• Epitope: C-KHQSLRPDEDSSHD
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005542.1; NP_001002231.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 8 ug/mL
  • Immunohistochemistry-Paraffin 2.5 - 8 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 2 ug/mL

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kallikrein 2 Antibody

• EC 3.4.21
• EC 3.4.21.35
• glandular kallikrein 2
• Glandular kallikrein-1
• hGK-1
• hK2
• Kallikrein 2
• kallikrein 2, prostatic
• kallikrein-2
• kallikrein-related peptidase 2
• Klk1c2
• KLK2
• KLK2A2
• MGC12201
• Tissue kallikrein-2
• Ton

Background

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 2 Antibody (NBP1-06046)(1)

Publication using NBP1-06046

• Stephan C, Xu C, Brown DA et al. Three new serum markers for prostate cancer detection within a percent free PSA-based artificial neural network. Prostate 2006-05-01 [PMID: 16388506]

Bioinformatics

• Gene Symbol: KLK2
• Entrez:
  • 3817(Human)
• Uniprot:
  • NP_005542.1(Human)