HES5 Antibody (HES5/8035)

Formalin-fixed, paraffin-embedded human colon stained with HES5 Antibody (HES5/8035). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: HES5/8035
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HES5 Antibody (HES5/8035) Summary

• Description: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Immunogen: Recombinant fragment of human HES5 protein (exact sequence is proprietary) (Uniprot: Q5TA89)
• Localization: Cell surface
• Isotype: IgG2 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HES5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for HES5 Antibody (HES5/8035)

• BHLHB38
• bHLHb38Class B basic helix-loop-helix protein 38
• hairy and enhancer of split 5 (Drosophila)
• Hairy and enhancer of split 5
• transcription factor HES-5

Background

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HES5