Hepsin Antibody (506230) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: IHC
• Clone: 506230
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Hepsin Antibody (506230) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Hepsin
  Ser46-Leu417
  Accession # P05981
• Specificity: Detects human Hepsin in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hepsin Antibody (506230) [Alexa Fluor® 700]

• EC 3.4.21
• EC 3.4.21.106
• Hepsin
• HPN
• serine protease hepsin catalytic chain
• serine protease hepsin
• TMPRSS1
• TMPRSS1serine protease hepsin non-catalytic chain
• Transmembrane protease serine 1
• transmembrane protease, serine 1

Background

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver but is also present in many other tissues; most notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII and may initiate blood coagulation at the cell surface (4). Hepsin is over-expressed in various human tumors including prostate (5) and is considered to be a biomarker for prostate cancer (6). Recombinant human (rh) Hepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the rhHepsin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.