Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (hippocampus) tissue. PVDF membrane ...read more
Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Gly184-Lys456 Accession # Q9Y661
Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs, and human and mouse HS3ST4 in Western blots. In Western blots, no cross-reactivity with recombinant human HS3ST3B1 is observed.
Protein A or G purified from hybridoma culture supernatant
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Heparan sulfate is a highly sulfated polysaccharide that can be found on
the cell surface and within the extracellular matrix. It is typically
covalently attached to the protein core of proteoglycans, such as
syndecans and glypicans. Heparin, on the other hand, can be considered
as a highly sulfated version of heparan sulfate that is predominantly
found in mast cells. Both heparin and heparan sulfate contain
disaccharide repeats of uronic acid and N‑acetylglucosamine and are
modified by the same sulfotransferases (1, 2). The uronic acid residues
can be sulfated at the 2-O position by heparan sulfate 2-O
sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be
sulfated at the N, 3-O, and 6-O positions by
N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O
sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases
(HS6STs) respectively. There are seven HS3STs in the human genome (3,
4). HS3ST4 and HS3ST2 are brain specific and may participate in
HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated
heparan sulfate disaccharide, the most highly sulfated sugar found in
biological samples (6, 7), and may have a role in assisting HSV-1 entry
and spread (8). HS3ST4 is a Golgi resident type II membrane protein and
has the longest proline rich stem region among all HS3STs (3, 5).
Bernfield, M. et al. (1999) Annu. Rev.
Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem.
Shworak, N.W. et al. (1999) J. Biol.
Xu, D. et al. (2005) Biochem. J.
Lawrence, R. et al. (2007) Matrix Biol.
Mochizuki, H. et al. (2003) J. Biol.
Wu, Z.L. et al. (2004) J. Biol. Chem.
Tiwari,V. et al. (2005) Biochem. Biophys.
Res. Commun. 338:930.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.