Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody (712011) [Alexa Fluor™ Plus 647]

• Clone: 712011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody (712011) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs, and human and mouse HS3ST4 in Western blots. In Western blots, no cross-reactivity with recombinant human HS3ST3B1 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody (712011) [Alexa Fluor™ Plus 647]

• 30ST4
• 3OST4 heparan sulfate 3-O-sulfotransferase-4
• 3-OST-4
• EC 2.8.2
• EC 2.8.2.23
• h3-OST-4
• heparan sulfate (glucosamine) 3-O-sulfotransferase 4
• Heparan sulfate 3-O-sulfotransferase 4
• Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4
• heparan sulfate glucosamine 3-O-sulfotransferase 4
• HS3ST4

Background

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5).

1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
4. Xu, D. et al. (2005) Biochem. J. 386:451.
5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.