HELZ Antibody (5B2) - Azide and BSA Free

Western Blot: HELZ Antibody (5B2) [H00009931-M02] - HELZ monoclonal antibody (M02), clone 5B2 Analysis of HELZ expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: HELZ Antibody (5B2) [H00009931-M02] - Analysis of monoclonal antibody to HELZ on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: HELZ Antibody (5B2) [H00009931-M02] - Analysis of monoclonal antibody to HELZ on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.

Sandwich ELISA: HELZ Antibody (5B2) [H00009931-M02] - Detection limit for recombinant GST tagged HELZ is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HELZ Antibody (5B2) - Azide and BSA Free Summary

• Immunogen: HELZ (NP_055692, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDRRAEKSCEQACESLKRQDYEMALKHCTEALLSLGQYSMADFTGPCPLEIERIKIESLLYRIASFLQLKNYVQADEDCRHVLGEGLAKGEDAFRAVLC
• Specificity: HELZ (5B2)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HELZ
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HELZ Antibody (5B2) - Azide and BSA Free

• DHRC
• DKFZp586G1924
• Down-regulated in human cancers protein
• DRHC
• EC 3.6.4.-
• helicase with zinc finger
• HUMORF5helicase with zinc finger domain
• KIAA0054MGC163454
• probable helicase with zinc finger domain

Background

HELZ is a member of the superfamily I class of RNA helicases. RNA helicases alter the conformation of RNA by unwinding double-stranded regions, thereby altering the biologic activity of the RNA molecule and regulating access to other proteins (Wagner et al., 1999 [PubMed 10471385]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HELZ Antibody (H00009931-M02)(1)

Publication using H00009931-M02

• Hasgall PA, Hoogewijs D, Faza MB et al. The Putative RNA Helicase HELZ Promotes Cell Proliferation, Translation Initiation and Ribosomal Protein S6 Phosphorylation. PLoS One. 2011-07-13 [PMID: 21765940]

Bioinformatics

• Gene Symbol: HELZ
• Entrez:
  • 9931(Human)
• OMIM:
  • 606699(Human)
• Uniprot:
  • NP_055692(Human)