Recombinant E. coli HDJ2 Protein

SDS-Page: HDJ2 Protein [NBC1-18361] - Dna J, 41.1kDa (376aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Ec
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant E. coli HDJ2 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-376 of E.coli HDJ2
  
  

  Source: E.coli

  Amino Acid Sequence: MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DNAJA1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 41.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris-HCl buffer (pH 8.8), 5 mM DTT, 100 mM NaCl, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant E. coli HDJ2 Protein

• dj-2
• DjA1
• DnaJ (Hsp40) homolog, subfamily A, member 1
• dnaJ homolog subfamily A member 1
• DnaJ protein homolog 2
• DNAJ2
• HDJ2
• hDJ-2
• Heat shock 40 kDa protein 4
• Heat shock protein J2
• heat shock protein, DNAJ-like 2
• HSDJ
• HSJ2
• HSJ-2
• HSPF4hdj-2
• Human DnaJ protein 2
• NEDD7
• neural precursor cell expressed, developmentally down-regulated 7

Background

DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70.. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. DnaJ(amino acids 1-376) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DNAJA1