HDAC9 Antibody (JU30-44)

Western Blot: HDAC9 Antibody (JU30-44) [NBP2-75519] - Western blot analysis of HDAC9 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: HDAC9 Antibody (JU30-44) [NBP2-75519] - Staining HDAC9 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HDAC9 Antibody (JU30-44) [NBP2-75519] - Analysis of paraffin-embedded human kidney tissue using anti-HDAC9 antibody. Counter stained with hematoxylin.

Western Blot: HDAC9 Antibody (JU30-44) [NBP2-75519] - Analysis of HDAC9 on different lysates using anti-HDAC9 antibody at 1/1,000 dilution.Positive control:Lane 1: K562 Lane 2: HepG2 Lane 3: Raji

Immunocytochemistry/Immunofluorescence: HDAC9 Antibody (JU30-44) [NBP2-75519] - Staining HDAC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HDAC9 Antibody (JU30-44) [NBP2-75519] - Staining HDAC9 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HDAC9 Antibody (JU30-44) [NBP2-75519] - Analysis of paraffin-embedded human tonsil tissue using anti-HDAC9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HDAC9 Antibody (JU30-44) [NBP2-75519] - Analysis of paraffin-embedded human colon cancer tissue using anti-HDAC9 antibody. Counter stained with hematoxylin.

Product Discontinued

HDAC9 Antibody (JU30-44) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human HDAC9 aa 1-50 / 1,011. (SwissProt: Q9UKV0 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HDAC9 Antibody (JU30-44)

• EC 3.5.1.98
• HD7b
• HD7HDAC
• HD9
• HDAC7
• HDAC7B
• HDAC7BDKFZp779K1053
• HDAC9
• HDRP
• histone deacetylase 4/5-related protein
• Histone deacetylase 7B
• histone deacetylase 9
• Histone deacetylase-related protein
• KIAA0744
• KIAA0744HDAC9B
• MEF-2 interacting transcription repressor (MITR) protein
• MEF2-interacting transcription repressor MITR
• MITR
• MITRHDAC9FL

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC9