HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free [NBP3-14060] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3 Antibody (PCRP-HDAC3-2D4).

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-HDAC3-2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13809).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human HDAC3 protein (Uniprot: O15379)
• Localization: Nucleus, Nucleoplasm, Golgi apparatus
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDAC3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order this antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for HDAC3 Antibody (PCRP-HDAC3-2D4) - Azide and BSA Free

• EC 3.5.1.98
• HD3RPD3-2RPD3
• HDAC3
• Histone Deacetylase 3
• KDAC3
• RPD3-2
• SMAP45

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC3