HDAC1 Antibody

Western Blot: HDAC1 Antibody [NBP2-16788] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP2-16788] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: HDAC1 Antibody [NBP2-16788] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [N1C1].

Product Discontinued

HDAC1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (100%), Rat (100%), Chicken (98%), Xenopus (97%), Zebrafish (97%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus tropicalis (97%), Drosophila (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for HDAC1 Antibody

• EC 3.5.1.98
• GON-10
• HD1
• HD1DKFZp686H12203
• Histone Deacetylase 1
• KDAC1
• reduced potassium dependency, yeast homolog-like 1
• RPD3L1
• RPD3L1RPD3

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HDAC1
• Uniprot:
  • Q13547(Human)