HCN1 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

HCN1 Antibody Summary

• Description: Novus Biologicals Sheep HCN1 Antibody (NBP1-20250) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-HCN1 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from cytoplasmic region of human HCN1 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HCN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:300-1:2000
  • Western Blot 1:300-1:2000
• Application Notes: Immunohistochemistry and Western Blot. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31923455).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for HCN1 Antibody

• BCNG1
• BCNG-1
• BCNG-1brain cyclic nucleotide gated channel 1
• Brain cyclic nucleotide-gated channel 1
• HAC-2
• HCN1
• hyperpolarization activated cyclic nucleotide-gated potassium channel 1
• potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Background

FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.; SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2. Interacts with KCNE2.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.; DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HCN1 Antibody (NBP1-20250)(6)

Publications using NBP1-20250

• Luo P, Fu X, Chang M et al. Cerebral ischemia-reperfusion causes a down regulation of HCN1 expression via enhancing the nuclear NRSF-HDAC4 gathering that contributes to neuron damage Brain Res. Bull. 2020-01-07 [PMID: 31923455] (ICC/IF, WB, Rat)
• Zhou M, Lin K, Si Y et al. Downregulation of HCN1 channels in hippocampus and prefrontal cortex in methamphetamine re-exposed mice with enhanced working memory Physiol Res 2018-10-23 [PMID: 30433806] (WB, Mouse)
• Luo P, Chen C, Lu Y et al. Baclofen ameliorates spatial working memory impairments induced by chronic cerebral hypoperfusion via up-regulation of HCN2 expression in the PFC in rats. Behav. Brain Res. 2016-04-13 [PMID: 27085590] (WB, Rat)
• Luo P, Lu Y, Li C et al. Long-lasting spatial learning and memory impairments caused by chronic cerebral hypoperfusion associate with a dynamic change of HCN1/HCN2 expression in hippocampal CA1 region. Neurobiol Learn Mem 2015-05-28 [PMID: 26021557]
• Zhou M, Luo P, Lu Y et al. Imbalance of HCn1 and HCn2 expression in hippocampal CA1 area impairs spatial learning and memory in rats with chronic morphine exposure. Prog. neuropsychopharmacol. Biol. Psychiatry. 2014-10-07 [PMID: 25301101] (WB, Rat)
• Li CJ, Lu Y, Zhou M et al. Activation of GABAB Receptors Ameliorates Cognitive Impairment via Restoring the Balance of HCN1/HCN2 Surface Expression in the Hippocampal CA1 Area in Rats With Chronic Cerebral Hypoperfusion. Mol. Neurobiol. 2014-05-18 [PMID: 24838625] (WB, Rat)

Bioinformatics

• Gene Symbol: HCN1
• Entrez:
  • 348980(Human)
  • 15165(Mouse)
  • 84390(Rat)
• Uniprot:
  • O60741(Human)