| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22450-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14084-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12563-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35653-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35270-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04110-20ul | |||
![Western Blot: HCN1 Antibody [NBP3-13014] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-13014-img0006.jpg "Western Blot: HCN1 Antibody [NBP3-13014] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HCN1 stained by HCN1 antibody (NBP3-13014) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13014-img0007.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0008.jpg)
![Western Blot: HCN1 Antibody [NBP3-13014] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-13014-img0001.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis. Sample: Paraffin-embedded mouse retina. HCN1 antibody (NBP3-13014) diluted at 1:800. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0002.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0003.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0004.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0005.jpg)
![Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Western-Blot-NBP1-22450-img0011.jpg "Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Neurons.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0014.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Human Hippocampus](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0001.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0012.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0013.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0015.jpg)
![Western Blot: HCN1 Antibody [NBP2-14084] - Analysis in human cell line WM-115.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP2-14084-img0003.jpg "Western Blot: HCN1 Antibody [NBP2-14084] - Analysis in human cell line WM-115.")
![HCN1 Antibody [NBP2-14084] -Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-25202313110..jpg)
![HCN1 Antibody [NBP2-14084] - Staining of human cerebellum shows strong positivity in neuronal processes in cells in granular layer.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-252023131231..jpg)
![HCN1 Antibody [NBP2-14084] -Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-252023131356..jpg)
![HCN1 Antibody [NBP2-14084] - Staining of human cervix, uterine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-252023131526..jpg)
![Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-12563-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563] - Staining of human HCN1 in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-12563-img0003.jpg)
![Immunoprecipitation: HCN1 Antibody [NBP3-12563] - Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-HCN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HCN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :100 kDa](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunoprecipitation-NBP3-12563-img0002.jpg)
![Western Blot: HCN1 Antibody [NBP3-35653] - Western blot analysis of various lysates, using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35653_rabbit-hcn1-pab-1012202411531095.jpg "Western Blot: HCN1 Antibody [NBP3-35653] - Western blot analysis of various lysates, using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunocytochemistry/ Immunofluorescence: HCN1 Antibody [NBP3-35653] - Immunofluorescence analysis of NIH/3T3 cells using HCN1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35653_rabbit-hcn1-pab-101220241224196.jpg)
![Western Blot: HCN1 Antibody [NBP3-35270] - Western blot analysis of various lysates using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35270_rabbit-hcn1-pab-101220241252854.jpg "Western Blot: HCN1 Antibody [NBP3-35270] - Western blot analysis of various lysates using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: HCN1 Antibody [NBP3-04110] - Analysis of extracts of various cell lines, using HCN1 antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-04110-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-04110] - Analysis of extracts of various cell lines, using HCN1 antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")