HCN1 Antibodies
We offer HCN1 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each HCN1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HCN1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the HCN1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HCN1 polyclonal antibodies and browse our HCN1 monoclonal antibody catalog.
Alternate Names for HCN1 Antibodies
anti-HCN1 antibody, anti-BCNG-1brain cyclic nucleotide gated channel 1 antibody, anti-BCNG1 antibody, anti-Brain cyclic nucleotide-gated channel 1 antibody, anti-HAC-2 antibody, anti-hyperpolarization activated cyclic nucleotide-gated potassium channel 1 antibody, anti-potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
9 Results for "hcn1" in Primary Antibodies
![Western Blot: HCN1 Antibody [NBP3-13014] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-13014-img0006.jpg "Western Blot: HCN1 Antibody [NBP3-13014] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HCN1 stained by HCN1 antibody (NBP3-13014) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13014-img0007.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0008.jpg)
![Western Blot: HCN1 Antibody [NBP3-13014] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-13014-img0001.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis. Sample: Paraffin-embedded mouse retina. HCN1 antibody (NBP3-13014) diluted at 1:800. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0002.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0003.jpg)
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HCN1 antibody (NBP3-13014) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-13014-img0004.jpg)
![Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Frozen-NBP3-13014-img0005.jpg)
![Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Western-Blot-NBP1-22450-img0011.jpg "Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Neurons.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0014.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Human Hippocampus](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0001.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0012.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0013.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0015.jpg)
![Western Blot: HCN1 Antibody [NBP2-14084] - Analysis in human cell line WM-115.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP2-14084-img0003.jpg "Western Blot: HCN1 Antibody [NBP2-14084] - Analysis in human cell line WM-115.")
![HCN1 Antibody [NBP2-14084] -Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-25202313110..jpg)
![HCN1 Antibody [NBP2-14084] - Staining of human cerebellum shows strong positivity in neuronal processes in cells in granular layer.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-252023131231..jpg)
![HCN1 Antibody [NBP2-14084] -Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-252023131356..jpg)
![HCN1 Antibody [NBP2-14084] - Staining of human cervix, uterine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/nbp2-14084_rabbit-polyclonal-hcn1-antibody-immunohistochemistry-paraffin-252023131526..jpg)
![Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-12563-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563] - Staining of human HCN1 in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunohistochemistry-Paraffin-NBP3-12563-img0003.jpg)
![Immunoprecipitation: HCN1 Antibody [NBP3-12563] - Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-HCN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HCN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :100 kDa](http://images.novusbio.com/fullsize/HCN1-Antibody-Immunoprecipitation-NBP3-12563-img0002.jpg)
![Western Blot: HCN1 Antibody [NBP3-35653] - Western blot analysis of various lysates, using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35653_rabbit-hcn1-pab-1012202411531095.jpg "Western Blot: HCN1 Antibody [NBP3-35653] - Western blot analysis of various lysates, using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunocytochemistry/ Immunofluorescence: HCN1 Antibody [NBP3-35653] - Immunofluorescence analysis of NIH/3T3 cells using HCN1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35653_rabbit-hcn1-pab-101220241224196.jpg)
![Western Blot: HCN1 Antibody [NBP3-35270] - Western blot analysis of various lysates using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35270_rabbit-hcn1-pab-101220241252854.jpg "Western Blot: HCN1 Antibody [NBP3-35270] - Western blot analysis of various lysates using HCN1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: HCN1 Antibody [NBP3-04110] - Analysis of extracts of various cell lines, using HCN1 antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP3-04110-img0001.jpg "Western Blot: HCN1 Antibody [NBP3-04110] - Analysis of extracts of various cell lines, using HCN1 antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: HCN1 Antibody [NBP2-22517] - Analysis of HCN1 in rat brain extracts.](http://images.novusbio.com/fullsize/HCN1-Antibody-Western-Blot-NBP2-22517-img0002.jpg "Western Blot: HCN1 Antibody [NBP2-22517] - Analysis of HCN1 in rat brain extracts.")