![Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-32518-img0007.jpg "Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit HCA59 Antibody - BSA Free (NBP1-32518) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HCA59. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | C9ORF78 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | C9ORF78 |
![Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-32518] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32518-img0003.jpg "Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-32518] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-32518] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-32518-img0004.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-32518] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.")
![Western Blot: HCA59 Antibody [NBP1-32518] - Sample(30 ug whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 12% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-32518-img0005.jpg "Western Blot: HCA59 Antibody [NBP1-32518] - Sample(30 ug whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 12% SDS PAGE, antibody diluted at 1:3000.")
![Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-32518-img0006.jpg "Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]](https://images.novusbio.com/images2/Synaptotagmin-1_MAB4364_Immunocytochemistry_11323.jpg)
![Immunohistochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]](https://images.novusbio.com/images2/Synaptotagmin-1_MAB4364_Immunohistochemistry_7025.jpg)
![Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]](https://images.novusbio.com/images2/Synaptotagmin1_MAB4364_Immunocytochemistry_13008.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")