HCA59 Antibodies
We offer HCA59 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each HCA59 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HCA59 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the HCA59 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HCA59 polyclonal antibodies.
Alternate Names for HCA59 Antibodies
anti-HCA59 antibody, anti-C9ORF78 antibody, anti-bA409K20.3 antibody, anti-chromosome 9 open reading frame 78 antibody, anti-Hepatocellular carcinoma-associated antigen 59 antibody, anti-HSPC220 antibody
10 Results for "hca59" in Primary Antibodies
![Western Blot: HCA59 Antibody [NBP1-83168] - Analysis using Anti-C9orf78 antibody NBP1-83168 (A) shows similar pattern to independent antibody NBP1-83170 (B).](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-83168-img0015.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HCA59 Antibody [NBP1-83168] - Analysis using Anti-C9orf78 antibody NBP1-83168 (A) shows similar pattern to independent antibody NBP1-83170 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-83168] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83168-img0008.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0025.jpg)
![Western Blot: HCA59 Antibody [NBP1-83168] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-83168-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HCA59 Antibody [NBP1-83168] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-C9orf78 antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-83168-img0012.jpg)
![Western Blot: HCA59 Antibody [NBP1-83168] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-83168-img0013.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human gall bladder shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0002.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0009.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0010.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human testis.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0016.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human kidney.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0017.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human lymph node.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Analysis in human bone marrow and pancreas tissues. Corresponding HCA59 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human bone marrow, fallopian tube, lymph node and testis using Anti-HCA59 antibody NBP1-83168 (A) shows similar protein distribution across tissues to independent antibody NBP1-83170 (B).](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0021.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP1-83168] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-NBP1-83168-img0022.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0023.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0024.jpg)
![Western Blot: HCA59 Antibody [NBP1-83170] - Analysis using Anti-C9orf78 antibody NBP1-83170 (A) shows similar pattern to independent antibody NBP1-83168 (B).](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-83170-img0015.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HCA59 Antibody [NBP1-83170] - Analysis using Anti-C9orf78 antibody NBP1-83170 (A) shows similar pattern to independent antibody NBP1-83168 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-83170] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83170-img0012.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0025.jpg)
![Western Blot: HCA59 Antibody [NBP1-83170] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-83170-img0013.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0006.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0009.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0010.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human kidney.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0016.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human testis.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0017.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human lymph node.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Analysis in human bone marrow and pancreas tissues. Corresponding HCA59 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human bone marrow, fallopian tube, lymph node and testis using Anti-HCA59 antibody NBP1-83170 (A) shows similar protein distribution across tissues to independent antibody NBP1-83169 (B).](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0021.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP1-83170] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-NBP1-83170-img0022.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0023.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83170] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83170-img0024.jpg)
![Western Blot: HCA59 Antibody [NBP1-83169] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probes #1 and #2, using Anti-C9orf78 antibody. The remaining relative intensity is presented.](http://images.novusbio.com/fullsize/antibody/nbp1-83169_rabbit-polyclonal-hca59-antibody-western-blot-1752023135346..jpg "Western Blot: HCA59 Antibody [NBP1-83169] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probes #1 and #2, using Anti-C9orf78 antibody. The remaining relative intensity is presented.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human bone marrow, fallopian tube, lymph node and testis using Anti-C9orf78 antibody NBP1-83169 (A) shows similar protein distribution across tissues to independent antibody NBP1-83170 (B).](http://images.novusbio.com/fullsize/nbp1-83169_rabbit-polyclonal-hca59-antibody-13520231625165.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human pancreas shows weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/nbp1-83169_rabbit-polyclonal-hca59-antibody-13520231625164.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Analysis in human bone marrow and pancreas tissues using NBP1-83169 antibody. Corresponding C9orf78 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/nbp1-83169_rabbit-polyclonal-hca59-antibody-13520231625162.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human cell line U-2 OS shows localization to the nucleoplasm.](http://images.novusbio.com/fullsize/antibody/nbp1-83169_rabbit-polyclonal-hca59-antibody-1752023132736.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/nbp1-83169_rabbit-polyclonal-hca59-antibody-1752023132830.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nbp1-83169_rabbit-polyclonal-hca59-antibody-175202313342024.jpg)
![Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-32518-img0007.jpg "Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-32518] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32518-img0003.jpg)
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-32518] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-32518-img0004.jpg)
![Western Blot: HCA59 Antibody [NBP1-32518] - Sample(30 ug whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 12% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-32518-img0005.jpg)
![Western Blot: HCA59 Antibody [NBP1-32518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HCA59 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HCA59-Antibody-Western-Blot-NBP1-32518-img0006.jpg)
![Immunohistochemistry: HCA59 Antibody [NBP3-06357] - Immunochemical staining of human HCA59 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-NBP3-06357-img0003.jpg "Immunohistochemistry: HCA59 Antibody [NBP3-06357] - Immunochemical staining of human HCA59 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry: HCA59 Antibody [NBP3-06357] - Immunochemical staining of human HCA59 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-NBP3-06357-img0002.jpg)
![HCA59 Antibody [NBP3-06357] - Anti-HCA59 rabbit polyclonal antibody at 1:500 dilutionLane A: H1299 Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDa](http://images.novusbio.com/fullsize/HCA59-Antibody-NBP3-06357-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP3-06357] - Immunofluorescence staining of HCA59 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06357 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06357_rabbit-polyclonal-hca59-antibody-235202516144340.png)
![Immunohistochemistry: HCA59 Antibody [NBP3-06353] - Immunochemical staining of human HCA59 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-NBP3-06353-img0002.jpg "Immunohistochemistry: HCA59 Antibody [NBP3-06353] - Immunochemical staining of human HCA59 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry: HCA59 Antibody [NBP3-06353] - Immunochemical staining of human HCA59 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HCA59-Antibody-Immunohistochemistry-NBP3-06353-img0001.jpg)
![Immunoprecipitation:HCA59 AntibodyNBP3-29011] -Detection of human C9orf78 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C9orf78 antibody NBP3-29011 (lot NBP3-29011-1) used for IP at 6 µg per reaction. C9orf78 was also immunoprecipitated by rabbit anti-C9orf78 antibody BL17619. For blotting immunoprecipitated C9orf78, NBP3-29011 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29011_rabbit-hca59-pab-301220241917287.jpg "Immunoprecipitation:HCA59 AntibodyNBP3-29011] -Detection of human C9orf78 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C9orf78 antibody NBP3-29011 (lot NBP3-29011-1) used for IP at 6 µg per reaction. C9orf78 was also immunoprecipitated by rabbit anti-C9orf78 antibody BL17619. For blotting immunoprecipitated C9orf78, NBP3-29011 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")