H-2Db Antibody (27-11-13S) [Biotin]

• Reactivity: Mu
• Applications: Flow, IHC, IHC-Fr, IHC-P, IP, ICC/IF
• Clone: 27-11-13S
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: 0.5 mg/ml

H-2Db Antibody (27-11-13S) [Biotin] Summary

• Immunogen: BDF1 mouse splenocytes
• Specificity: An epitope on the alpha chain of H-2D
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2-D1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/10^6 cells
  • Immunocytochemistry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for H-2Db Antibody (27-11-13S) [Biotin]

• H2Db
• MHC class I

Background

MAb 27-11-13 recognizes with the alpha 3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha 3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2-D1