GULP1/CED-6 Antibody

Western Blot: GULP1/CED-6 Antibody [NB100-1042] - Analysis of GULP1 in Human Placenta lysate (RIPA buffer, 30ug total protein per lane) using NB100-1042 at 0.2ug/ml. Primary incubated for 1 hour. Detected using chemiluminescence.

Immunohistochemistry-Paraffin: GULP1/CED-6 Antibody [NB100-1042] - Analysis of GULP1 in paraffin embedded Human Kidney using NB100-1042 at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GULP1/CED-6 Antibody Summary

• Description: Novus Biologicals Goat GULP1/CED-6 Antibody (NB100-1042) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-GULP1/CED-6 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EGTVFCLDPLDSRC corresponding to C-Terminus according to NP_057399.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GULP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.2 - 0.6 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human placenta lysates (calculated MW of 34.5 kDa band according to NP_057399.1). An additional band of unknown identity was also consistently observed at approx.46 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GULP1/CED-6 Antibody

• CED6
• CED6GULPCED-6
• Cell death protein 6 homolog
• engulfment adapter protein
• FLJ31156
• GULP, engulfment adaptor PTB domain containing 1
• GULP1
• Protein GULP
• PTB domain adapter protein CED-6
• PTB domain adaptor protein CED-6
• PTB domain-containing engulfment adapter protein 1

Background

The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, as well as inflammation and autoimmunity. CED6 and its human homologue gulp, encode an adapter protein, whose function in engulfment. The upstream and downstream components of CED6 mediated signaling are not known. Recently, CED1 has been shown to encode a transmembrane protein on phagocytic cells, with two functional sequence motifs in its cytoplasmic tail that are important for engulfment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GULP1/CED-6 Antibody (NB100-1042)(3)

Publications using NB100-1042

• Faralli JA, Desikan H, Peotter J et al. Genomic/proteomic analyses of dexamethasone-treated human trabecular meshwork cells reveal a role for GULP1 and ABR in phagocytosis Mol. Vis. 2019-04-25 [PMID: 31516309]
• Jha A, Watkins SC, Traub LM. The apoptotic engulfment protein Ced-6 participates in clathrin-mediated yolk uptake in Drosophila egg chambers. Mol Biol Cell. 2012-05-01 [PMID: 22398720]
  
  Details:
  Fig 9D (purified rat placenta and brain clathrin-coated vesicles, rat placenta and brain cytosol). GULP was detected at ~35 kDa in placenta cytosol and strongly enriched in clathrin-coated vesicles purified from placenta. GULP was not detected in brain cy
• Su HP, Nakada-Tsukui K, Tosello-Trampont AC et al. Interaction of CED-6/GULP, an adapter protein involved in engulfment ofapoptotic cells with CED-1 and CD91/low density lipoprotein receptor-relatedprotein (LRP). J Biol Chem 2002-04-05 [PMID: 11729193]

FAQs for GULP1/CED-6 Antibody (NB100-1042). (Showing 1 - 1 of 1 FAQs).

1. Is GULP1 localized to mitochondria?
   • I am showing based on localization data from UniProt that it may associate with the cytoplasmic side of the plasma membrane: http://www.uniprot.org/uniprot/Q9UBP9 Additional localization data may be found here at Human protein atlas: http://www.proteinatlas.org/ENSG00000144366

Bioinformatics

• Gene Symbol: GULP1
• Entrez:
  • 51454(Human)
  • 70676(Mouse)
• Uniprot:
  • Q9UBP9(Human)