GULP1/CED-6 Antibody

Images

 
Western Blot: GULP1/CED-6 Antibody [NB100-1042] - Analysis of GULP1 in Human Placenta lysate (RIPA buffer, 30ug total protein per lane) using NB100-1042 at 0.2ug/ml. Primary incubated for 1 hour. Detected using ...read more
Immunohistochemistry-Paraffin: GULP1/CED-6 Antibody [NB100-1042] - Analysis of GULP1 in paraffin embedded Human Kidney using NB100-1042 at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GULP1/CED-6 Antibody Summary

Immunogen
Peptide with sequence EGTVFCLDPLDSRC corresponding to C-Terminus according to NP_057399.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GULP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 35 kDa band observed in human placenta lysates (calculated MW of 34.5 kDa band according to NP_057399.1). An additional band of unknown identity was also consistently observed at approx.46 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules.
Publications
Read Publications using NB100-1042.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GULP1/CED-6 Antibody

  • CED6
  • CED6GULPCED-6
  • Cell death protein 6 homolog
  • engulfment adapter protein
  • FLJ31156
  • GULP, engulfment adaptor PTB domain containing 1
  • GULP1
  • Protein GULP
  • PTB domain adapter protein CED-6
  • PTB domain adaptor protein CED-6
  • PTB domain-containing engulfment adapter protein 1

Background

The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, as well as inflammation and autoimmunity. CED6 and its human homologue gulp, encode an adapter protein, whose function in engulfment. The upstream and downstream components of CED6 mediated signaling are not known. Recently, CED1 has been shown to encode a transmembrane protein on phagocytic cells, with two functional sequence motifs in its cytoplasmic tail that are important for engulfment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GULP1/CED-6 Antibody (NB100-1042)(2)

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Video Protocols

WB Video Protocol

FAQs for GULP1/CED-6 Antibody (NB100-1042). (Showing 1 - 1 of 1 FAQs).

  1. Is GULP1 localized to mitochondria?
    • I am showing based on localization data from UniProt that it may associate with the cytoplasmic side of the plasma membrane: http://www.uniprot.org/uniprot/Q9UBP9 Additional localization data may be found here at Human protein atlas: http://www.proteinatlas.org/ENSG00000144366

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GULP1
Entrez
Uniprot