GSTO1 Antibody (2C7) - BSA Free

Western Blot: GSTO1 Antibody (2C7) [NBP3-15189] - Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: GSTO1 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 24, 25 KDa. Observed band size: 28 KDa.

Immunocytochemistry/Immunofluorescence: GSTO1 Antibody (2C7) [NBP3-15189] - Immunofluorescence staining of HepG2 cells with at GSTO1 antibody 1:41, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: GSTO1 Antibody (2C7) [NBP3-15189] - IHC image of GSTO1 antibody diluted at 1:125 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry: GSTO1 Antibody (2C7) [NBP3-15189] - IHC image of GSTO1 antibody diluted at 1:125 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2C7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GSTO1 Antibody (2C7) - BSA Free Summary

• Description: Novus Biologicals Rabbit GSTO1 Antibody (2C7) - BSA Free (NBP3-15189) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human GSTO1. UniProt ID (P78417).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSTO1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GSTO1 Antibody (2C7) - BSA Free

• DKFZp686H13163
• EC 2.5.1.18
• glutathione S-transferase omega 1
• glutathione S-transferase omega 1-1
• glutathione S-transferase omega-1
• glutathione-S-transferase like
• GSTO-1
• GSTTLp28
• P28

Background

GSTO1 encodes a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, the encoded protein acts as a small stress response protein, likely involved in cellular redox homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTO1