GSK-3 alpha Antibody

Images

 
Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from ovary cancer cells using GSK3 alpha (Ab-21) antibody.
Immunocytochemistry/ Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells using GSK3a(Ab-21) antibody.
Immunohistochemistry: GSK-3 alpha Antibody [NB100-81943] - Analysis of paraffin- embedded human breast carcinoma tissue using GSK3 alpha (Ab-21) antibody.
Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from 293 cells using GSK3a(Ab-21) antibody.
Immunoprecipitation: GSK-3 alpha Antibody [NB100-81943] - Human HaCaT keratinocyte lysates with GSK3 alpha Antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GSK-3 alpha Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human GSK3 alpha around a.a 21 (T-S-S-F-A). IP data from customer review.
Specificity
GSK3 alpha (specific to a.a. 21) detects endogenous levels of total GSK3 alpha protein. Does not detect GSK-3b protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100~1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
GSK-3 alpha Lysate (NBL1-11361)
Reviewed Applications
Read 1 Review rated 5
using
NB100-81943 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSK-3 alpha Antibody

  • DKFZp686D0638
  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 alpha
  • glycogen synthase kinase-3 alpha
  • GSK3 alpha
  • GSK-3 alpha
  • GSK3A

Background

Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 alpha Antibody (NB100-81943) (0)

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Review for GSK-3 alpha Antibody (NB100-81943) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using NB100-81943:
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Images Ratings Applications Species Date Details
Immunoprecipitation GSK-3 alpha NB100-81943
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reviewed by:
Leeni Koivisto
IP Human 07/14/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedHaCaT keratinocyte whole cell lysate
SpeciesHuman
Lot5117
CommentsGSK3 alpha Antibody [NB100-81943] immunoprecipitates human cell lysates very well and was specific to the alpha isoform, as demonstrated by the absence of the beta isoform in the immunoprecipitate

Blocking

Blocking DetailsLi-Cor Odyssey blocking buffer, 1 h at RT

Primary Anitbody

Dilution RatioNon-Novus antibody that recognizes both GSK3 alpha and beta

Secondary Antibody

Secondary DescriptionGoat anti-mouse IRDye800
Secondary Concentration1/15,000

Details

Detection NotesLi-Cor Odyssey intrared reader, whole cell lysate was used as a positive control for the immunoprecipitation
Immunoprecipitation DetailsDynabeads protein G 1.5 mg, incubation o/n at +4ºC, washed 5 times with TBS-Tween 20
Elution Description0.1 M Glycine-HCl pH 2.8, 10 min at RT

Comments

CommentsGSK3 alpha Antibody [NB100-81943] immunoprecipitates human cell lysates very well and was specific to the alpha isoform, as demonstrated by the absence of the beta isoform in the immunoprecipitate

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GSK-3 alpha Antibody (NB100-81943) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Leeni Koivisto
07/14/2014
Application: IP
Species: Human

Bioinformatics

Gene Symbol GSK3A
Entrez
Uniprot