GSK-3 alpha Antibody

Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from ovary cancer cells using GSK3 alpha (Ab-21) antibody.

Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells using GSK3a(Ab-21) antibody.

Immunohistochemistry: GSK-3 alpha Antibody [NB100-81943] - Analysis of paraffin- embedded human breast carcinoma tissue using GSK3 alpha (Ab-21) antibody.

Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from 293 cells using GSK3a(Ab-21) antibody.

Immunoprecipitation: GSK-3 alpha Antibody [NB100-81943] - Human HaCaT keratinocyte lysates with GSK3 alpha Antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, IP, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

GSK-3 alpha Antibody Summary

• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human GSK-3 alpha around amino acids 19~23 (T-S-S-F-A). IP data from customer review.
• Specificity: Detects endogenous levels of total GSK-3 alpha protein. Does not detect GSK-3 beta protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSK3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100~1:200
  • Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  GSK-3 alpha Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GSK-3 alpha Antibody

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK3 alpha
• GSK-3 alpha
• GSK3A

Background

Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSK-3 alpha Antibody (NB100-81943)(1)

Publication using NB100-81943

• Davulur, G, Giusto M, Chandel R et al. Impaired Ribosomal Biogenesis by Non-Canonical Degradation of beta-catenin during Hyperammonemia Mol. Cell. Biol. 2019-06-17 [PMID: 31138664]

Review for GSK-3 alpha Antibody (NB100-81943) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IP Human 07/14/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: HaCaT keratinocyte whole cell lysate
• Species: Human
• Lot: 5117

Bioinformatics

• Gene Symbol: GSK3A
• Entrez:
  • 2931(Human)
  • 50686(Rat)
• Uniprot:
  • P49840()