GSK-3 alpha Antibody (S07-3A2)

Western Blot: GSK-3 alpha Antibody (S07-3A2) [NBP3-15036] - Western blot detection of GSK-3 alpha in K562, Rat Brain, Hela cell lysates using GSK-3 alpha Rabbit mAb (1:1000 diluted). Predicted band size: 51kDa. Observed band size: 51kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S07-3A2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GSK-3 alpha Antibody (S07-3A2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human GSK-3 alpha (Uniprot#: P49840)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSK3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for GSK-3 alpha Antibody (S07-3A2)

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK3 alpha
• GSK-3 alpha
• GSK3A

Background

Glycogen synthases kinase 3 alpha (GSK3-alpha) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-alpha activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-alpha by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-alpha activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3A