GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) - BSA Free

Immunohistochemistry: GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) [NBP3-15149] - IHC image of GSK-3 alpha antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minute at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 4A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) - BSA Free Summary

• Description: Novus Biologicals Rabbit GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) - BSA Free (NBP3-15149) is a recombinant monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human Phospho-GSK-3 alpha (Y216 + Y279). UniProt ID (P49840).
• Modification: p Tyr216, p Tyr279
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSK3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) - BSA Free

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK3 alpha
• GSK-3 alpha
• GSK3A

Background

Glycogen synthases kinase 3 alpha (GSK3-alpha) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-alpha activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-alpha by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-alpha activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3A