GRWD1 Antibody

Western Blot: GRWD1 Antibody [NBP1-81790] - Analysis using Anti-GRWD1 antibody NBP1-81790 (A) shows similar pattern to independent antibody NBP2-56888 (B).

Immunocytochemistry/Immunofluorescence: GRWD1 Antibody [NBP1-81790] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.

Immunohistochemistry-Paraffin: GRWD1 Antibody [NBP1-81790] - Staining of human lymph node shows nuclear positivity.

Product Discontinued

GRWD1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PVTSVEWHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKELHWHPQCPGLLVSTALSGFTIFRTISV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRWD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GRWD1 Antibody

• CDW4
• CUL4- and DDB1-associated WDR protein 4
• glutamate-rich WD repeat containing 1
• GRWDglutamate rich WD repeat protein GRWD
• KIAA1942regulator of ribosome biogenesis 1 homolog
• RRB1
• WD repeat domain 28
• WDR28glutamate-rich WD repeat-containing protein 1

Background

GRWD1 is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis (Gratenstein et al., 2005 [PubMed 15885502]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRWD1