BOP1 Antibody

Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389] - FFPE section of human colon carcinoma (left) and mouse teratoma. Antibody:Affinity purified rabbit anti-BOP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: BOP1 Antibody [NBP1-21389] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-21388 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

BOP1 Antibody Summary

• Description: Novus Biologicals Rabbit BOP1 Antibody (NBP1-21389) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human block of proliferation 1 using the numbering given in entry NP_056016.1 (GeneID 23246).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BOP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BOP1 Antibody

• block of proliferation 1
• KIAA0124Block of proliferation 1 protein
• ribosome biogenesis protein BOP1

Background

BOP1 (block of proliferation 1) is a component of the PeBoW (Pes1, Bop, WDR12) complex that plays a role in the processing of 28S and 5.8S ribosomal RNAs and the formation of the 60S ribosome. BOP1 contains seven WD repeats and was originally identified as a cDNA which inhibited cell proliferation. The deregulation of the BOP1 gene is proposed to contribute to colorectal tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BOP1
• Entrez:
  • 23246(Human)
• Uniprot:
  • NP_056016.1(Human)