GRP75/HSPA9B/Mortalin Antibody (S52A-42)

Western Blot: GRP75/HSPA9B/Mortalin Antibody (S52A-42) [NBP1-21670] - analysis of Human HeLa cell lysates showing detection of Grp75 protein using Mouse Anti-Grp75 Monoclonal Antibody, Clone S52A-42 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (S52A-42) [NBP1-21670] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondria. Magnification: 100x.

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (S52A-42) [NBP1-21670] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondria. Magnification: 20x.

Product Discontinued

GRP75/HSPA9B/Mortalin Antibody (S52A-42) Summary

• Immunogen: Fusion protein amino acids 551-766 of mouse SALM2.
• Localization: Mitochondrion
• Marker: Immortalization Marker
• Specificity: Detects approx 75kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: 1 ug/ml was sufficient for detection of Grp75 in 10 ug of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GRP75/HSPA9B/Mortalin Antibody (S52A-42)

• 75 kDa glucose-regulated protein
• CSA
• GRP75
• GRP-75
• GRP75mthsp75
• Heat shock 70 kDa protein 9
• heat shock 70kD protein 9B
• heat shock 70kDa protein 9 (mortalin)
• heat shock 70kDa protein 9B (mortalin-2)
• HSPA9
• HSPA9B
• HSPA9BMGC4500
• Mortalin
• mortalin, perinuclear
• Mortalin-2
• MOT
• MOT2
• mot-2
• MTHSP75
• p66-mortalin
• PBP74
• PBP74CSA
• Peptide-binding protein 74
• stress-70 protein, mitochondrial

Background

Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle (3, 4). Related forms may also be found in the cytosol or on the surface of the extracellular membrane. Other Grp75 functions include its ability to inactivate the tumor suppressor p53 (5). Studies have found that Grp75 is ver-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (6). Grp75 is also implicated in cell aging, as its over expression appears to prolong the life span of human fibroblasts (7). And finally, like its E.coli homolog DnaK (8), GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone (9, 10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA9
• Entrez:
  • 3313(Human)
• Uniprot:
  • P38646()