GRHL2 Antibody

Western Blot: GRHL2 Antibody [H00079977-B01P] - Analysis of GRHL2 expression in A-431.

Immunocytochemistry/Immunofluorescence: GRHL2 Antibody [H00079977-B01P] - Analysis of purified antibody to GRHL2 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: GRHL2 Antibody [H00079977-B01P] - Analysis of GRHL2 expression in transfected 293T cell line by GRHL2 polyclonal antibody. Lane 1: GRHL2 transfected lysate(68.75 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

GRHL2 Antibody Summary

• Immunogen: GRHL2 (NP_079191.1, 1 a.a. - 625 a.a.) full-length human protein. MSQESDNNKRLVALVPMPSDPPFNTRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRDKRLLSVSKASDSQEDQEKRNCLGTSEAQSNLSGGENRVQVLKTVPVNLSLNQDHLENSKREQYSISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYPRGDGEEQRVVIFEQTQYDVPSLATHSAYLKDDQRSTPDSTYSESFKDAATEKFRSASVGAEEYMYDQTSSGTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGDNKCFRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKGQASQTQCNSSSDGKLAAIPLQKKSDITYFKTMPDLHSQPVLFIPDVHFANLQRTGQVYYNTDDEREGGSVLVKRMFRPMEEEFGPVPSKQMKEEGTKRVLLYVRKETDDVFDALMLKSPTVKGLMEAISEKYGLPVEKIAKLYKKSKKGILVNMDDNIIEHYSNEDTFILNMESMVEGFKVTLMEI
• Specificity: GRHL2 - grainyhead-like 2 (Drosophila),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GRHL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GRHL2 Antibody

• BOMMGC149294
• Brother of mammalian grainyhead
• deafness, autosomal dominant 28
• DFNA28
• FLJ13782
• grainyhead-like 2 (Drosophila)
• grainyhead-like protein 2 homolog
• TFCP2L3MGC149295
• Transcription factor CP2-like 3FLJ11172

Background

TFCP2L3 is a member of a family of transcription factor genes whose archetype is TFCP2 (MIM 189889), a mammalian ortholog of the Drosophila gene 'grainyhead' (grh).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRHL2 Antibody (H00079977-B01P)(2)

Publications using H00079977-B01P

• Mehrazarin S, Chen W, Oh JE et al. p63 Gene is Regulated by Grainyhead-Like 2 (GRHL2) Through Reciprocal Feedback and Determines Epithelial Phenotype in Human Keratinocytes. J Biol Chem. 2015-06-17 [PMID: 26085095]
• Dompe N, Rivers CS, Li L et al. A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis. Proc Natl Acad Sci U S A. 2011-09-26 [PMID: 21949371]

Bioinformatics

• Gene Symbol: GRHL2
• Entrez:
  • 79977(Human)
• Uniprot:
  • NP_079191.1(Human)