GRB7 Antibody

Western Blot: GRB7 Antibody [NB100-1027] - Antibody at 1ug/ml. Staining of A431 lysate (35u g protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GRB7 Antibody Summary

• Immunogen: Peptide with sequence ELDLSPPHLSSSPE-C corresponding to N-Terminus according to NP_005301.2.
• Specificity: This antibody recognizes the longer isoform of this protein in A431 lysate.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRB7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/mL
• Application Notes: Western blot: Approx 65 kDa band observed in lysates of cell line A431 (calculated MW of 59.7 kDa according to NP_005301.2).
• Control:

  A-431 Whole Cell Lysate
  GRB7 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRB7 Antibody

• B47
• Epidermal growth factor receptor GRB-7
• GRB7 adapter protein
• GRB7
• growth factor receptor-bound protein 7

Background

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRB7 Antibody (NB100-1027)(1)

Publication using NB100-1027

• Margolis B, Silvennoinen O, Comoglio F et al. High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains. Proc Natl Acad Sci U S A 1992-10-01 [PMID: 1409582]

Bioinformatics

• Gene Symbol: GRB7
• Entrez:
  • 2886(Human)
  • 14786(Mouse)
• Uniprot:
  • NP_005301.(Human)