Granzyme K Antibody (2471A) [Unconjugated]

HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (<a class=

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Granzyme K was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

Granzyme K was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (<a class=

CCM032). NK cells were stained with (A) Mouse Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216), followed by PE-conjugated anti-Rabbit IgG secondary antibody (F0110) and Rabbit Anti-Human CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R). (B) Quadrant markers were set based on control antibody staining (MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." > CCM032). NK cells were stained with (A) Mouse Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216), followed by PE-conjugated anti-Rabbit IgG secondary antibody (F0110) and Rabbit Anti-Human CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R). (B) Quadrant markers were set based on control antibody staining (MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." title="Detection of Granzyme K in expanded Human NK cells by Flow Cytometry. NK cells were expanded from Human peripheral blood mononuclear cells (PBMCs) for 14 days using ExCellerate™ Human NK Cell Expansion Media (CCM032). NK cells were stained with (A) Mouse Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216), followed by PE-conjugated anti-Rabbit IgG secondary antibody (F0110) and Rabbit Anti-Human CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R). (B) Quadrant markers were set based on control antibody staining (MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." />

Detection of Granzyme K in expanded Human NK cells by Flow Cytometry. NK cells were expanded from Human peripheral blood mononuclear cells (PBMCs) for 14 days using ExCellerate™ Human NK Cell Expansion Media (<a class=

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: 2471A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Granzyme K Antibody (2471A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing human Granzyme K
  Accession # P49863
• Specificity: Detects human Granzyme K in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme K Antibody (2471A) [Unconjugated]

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• Fragmentin-3
• Granzyme 3
• granzyme K (granzyme 3; tryptase II)
• granzyme K (serine protease, granzyme 3; tryptase II)
• Granzyme K
• Granzyme-3
• Granzyme-K
• GZMK
• NK-Tryp-2
• NK-Tryptase-2
• PRSS
• TRYP2
• TRYP2granzyme-3
• Tryptase II

Background

Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells.  They are serine proteases that induce apoptosis in the target cell.  Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state.  Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.

1. Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
2. Walch, M. et al. (2014). Cell. 157:1309.
3. Cullen, SP. et al. (2010). Cell Death Differ. 17:616.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme K Antibody (MAB10216)(1)

Publication using MAB10216

• Aubert, A;Liu, A;Kao, M;Goeres, J;Richardson, KC;Nierves, L;Jung, K;Nabai, L;Zhao, H;Orend, G;Krawetz, R;Lange, PF;Younger, A;Chan, J;Granville, DJ; Granzyme B cleaves Tenascin-C to release its C-terminal domain in rheumatoid arthritis JCI insight 2024-10-17 [PMID: 39475853] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • P49863()