Granzyme K Antibody (2471A) [Alexa Fluor® 594]

202-IL), Recombinant Human IL-12 Protein (219-IL), Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with Rabbit Anti-Human NCAM-1/CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R) and either (A) Rabbit Anti-Human Granzyme K Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC10216T) or (B) Normal Rabbit IgG Control (AB-105-C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." > 202-IL), Recombinant Human IL-12 Protein (219-IL), Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with Rabbit Anti-Human NCAM-1/CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R) and either (A) Rabbit Anti-Human Granzyme K Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC10216T) or (B) Normal Rabbit IgG Control (AB-105-C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." title="NK cells treated with Recombinant Human IL-2 Protein (202-IL), Recombinant Human IL-12 Protein (219-IL), Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with Rabbit Anti-Human NCAM-1/CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R) and either (A) Rabbit Anti-Human Granzyme K Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC10216T) or (B) Normal Rabbit IgG Control (AB-105-C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." />

NK cells treated with Recombinant Human IL-2 Protein (<a class=

• Applications: Flow
• Clone: 2471A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 594

Granzyme K Antibody (2471A) [Alexa Fluor® 594] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide containing human Granzyme K
  Accession # P49863
• Specificity: Detects human Granzyme K in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme K Antibody (2471A) [Alexa Fluor® 594]

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• Fragmentin-3
• Granzyme 3
• granzyme K (granzyme 3; tryptase II)
• granzyme K (serine protease, granzyme 3; tryptase II)
• Granzyme K
• Granzyme-3
• Granzyme-K
• GZMK
• NK-Tryp-2
• NK-Tryptase-2
• PRSS
• TRYP2
• TRYP2granzyme-3
• Tryptase II

Background

Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells.  They are serine proteases that induce apoptosis in the target cell.  Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state.  Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.

1. Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
2. Walch, M. et al. (2014). Cell. 157:1309.
3. Cullen, SP. et al. (2010). Cell Death Differ. 17:616.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P49863()