Granzyme H Antibody [Biotin]

Granzyme H was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Granzyme H Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Granzyme H Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Granzyme H
  Glu19-Leu246
  Accession # P20718
• Specificity: Detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Granzyme C, rmGranzyme D, and rmGranzyme G is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GZMH
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme H Antibody [Biotin]

• Cathepsin G-like 2
• CCP-X
• CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1
• CGL2
• CGL-2
• CSP-Ccytotoxin serine protease-C
• CTLA1
• CTSGL2
• CTSGL2granzyme H
• Cytotoxic serine protease C
• Cytotoxic T-lymphocyte proteinase
• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.79
• granzyme H (cathepsin G-like 2, protein h-CCPX)
• Granzyme H
• GrzH
• GZMH

Background

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). The pro-enzyme is expressed and purified. After being activated by active cathepsin C, rhGranzyme H cleaves a thioester substrate, which was described previously (8).

1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
3. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
4. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
5. Meier, M. et al. (1990) Biochemistry 29:4042.
6. Haddad, P. et al. (1991) Int. Immunol. 3:57.
7. Klein, J.L. et al. (1990) Tissue Antigens 35:220.
8. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme H Antibody (BAF1377)(1)

Publication using BAF1377

• HJ Park, MS Shin, M Kim, JB Bilsborrow, S Mohanty, RR Montgomery, AC Shaw, S You, I Kang Transcriptomic analysis of human IL-7 receptor alpha low and high effector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults Aging Cell, 2019-05-01;0(0):e12960. 2019-05-01 [PMID: 31044512] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: GZMH
• Uniprot:
  • P20718()