Granzyme H Antibody [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 669

Granzyme H Antibody [Janelia Fluor® 669] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Granzyme H
  Glu19-Leu246
  Accession # P20718
• Specificity: Detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Granzyme C, rmGranzyme D, and rmGranzyme G is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GZMH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Granzyme H Antibody [Janelia Fluor® 669]

• Cathepsin G-like 2
• CCP-X
• CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1
• CGL2
• CGL-2
• CSP-Ccytotoxin serine protease-C
• CTLA1
• CTSGL2
• CTSGL2granzyme H
• Cytotoxic serine protease C
• Cytotoxic T-lymphocyte proteinase
• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.79
• granzyme H (cathepsin G-like 2, protein h-CCPX)
• Granzyme H
• GrzH
• GZMH

Background

The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GZMH