GRAIL/RNF128 Antibody

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Western Blot: GRAIL/RNF128 Antibody [NBP1-69426] - This Anti-RNF128 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1.25ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GRAIL/RNF128 Antibody Summary

Immunogen
Synthetic peptides corresponding to RNF128(ring finger protein 128) The peptide sequence was selected from the C terminal of RNF128. Peptide sequence LEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREI.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF128
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against RNF128 and was validated on Western blot.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for GRAIL/RNF128 Antibody

  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ23516
  • Gene related to anergy in lymphocytes protein
  • GRAIL
  • ring finger protein 128GRAILE3 ubiquitin-protein ligase RNF128
  • RNF128

Background

RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RNF128
Entrez
Uniprot