GPR83 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GPR83 Antibody [NLS4954] - Analysis of anti-GPR83 antibody with fresh prepared (top panel) and activated (bottom panel) cells stained with GPR83 antibody and PE-labeled FOXP3 antibody. Red is FOXP3 stained cells, Green is GPR83, and yellow are merged. Images courtesy of Dr. Sakagu.

Immunohistochemistry-Paraffin: GPR83 Antibody [NLS4954] - Analysis of anti-GPR83 antibody with human brain, cortex, neurons at dilution 9-18 ug/ml.

Flow Cytometry: GPR83 Antibody [NLS4954] - Analysis of anti-GPR83 antibody with purified spleen and lymph nodes stained with GPR83 antibody followed by Alexa488-anti-rabbit IgG. Cells were then stained with APC-anti-CD4 together with PE-anti-CD25 or with PE-anti-FOXP3. Gray is second antibody only and line.

• Reactivity: Hu, Po, Bv, Eq, Pm, Pm, Rb, Bt
• Applications: Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPR83 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 13 amino acid peptide from 3rd extracellular domain of human GPR83.
• Epitope: 3rd extracellular domain
• Localization: Integral membrane protein.
• Specificity: Human GPR83. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Bat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR83
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 9 - 18 ug/ml
• Application Notes: Use in Flow-cell surface reported in scientific literature (PMID: 16772372)..
• Control:

  GPR83 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Marmoset (100%), Mouse (85%), Rat (85%), Hamster (85%), Opossum (85%), Xenopus (85%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR83 Antibody - BSA Free

• G protein-coupled receptor 72
• G protein-coupled receptor 83
• GIR
• GPR72
• GPR72glucocorticoid induced receptor
• GPR83
• G-protein coupled receptor 72
• KIAA1540
• probable G-protein coupled receptor 83
• RP39
• RP82

Background

GPR72 is an Orphan-A GPCR with an unknown ligand. GPR72 has been reported in various regions of the brain. ESTs have been isolated from brain libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR83 Antibody (NLS4954)(1)

Publication using NLS4954

• Sugimoto N, Oida T, Hirota K et al. Foxp3-dependent and -independent molecules specific for CD25+CD4+ natural regulatory T cells revealed by DNA microarray analysis. Int Immunol. 2006-08-01 [PMID: 16772372] (Flow -CS)

Bioinformatics

• Gene Symbol: GPR83
• Entrez:
  • 10888(Human)