GPR109B/HM74 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody [NLS179] - Analysis of anti-GPR109B / HM74 antibody with human adipocytes at dilution 3-4 ug/ml.

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody [NLS179] - Analysis of anti-GPR109B / HM74 antibody with human spleen.

• Reactivity: Hu, Mu, Pm
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPR109B/HM74 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human GPR109B/HM74.
• Epitope: N-Terminus extracellular domain
• Localization: Multi-pass membrane protein
• Specificity: Human GPR109B/HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100%).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HCAR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 - 4 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 17008871)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR109B/HM74 Antibody - BSA Free

• G protein-coupled receptor 109B
• GPR109B
• G-protein coupled receptor 109B
• G-protein coupled receptor HM74
• G-protein coupled receptor HM74B
• GTP-binding protein
• HCA3
• HCAR3
• HM74
• HM74B
• HM74Puma-g
• Niacin receptor 2
• NIACR2
• Nicotinic acid receptor 2
• PUMAG
• putative chemokine receptor

Background

HM74 acts as a low affinity receptor for nicotinic acid and mediates its anti-lipolytic effect through a G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Expression in humans is largely restricted to adipose tissue and spleen but there are some reports of it being expressed in lymphocytes and monocytes. In mouse is expressed in lungs, spleen, heart, skeletal muscle and adipose tissue. In rat it is expressed in adipose tissue, lung and spleen. ESTs have been isolated from adipose, blood, liver, liver/spleen, placenta, prostate, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR109B/HM74 Antibody (NLS179)(1)

Publication using NLS179

• Maciejewski-Lenoir D, Richman JG, Hakak Y et al. Langerhans cells release prostaglandin D2 in response to nicotinic acid. J Invest Dermatol. 2006-12-01 [PMID: 17008871] (ICC/IF, Human, Mouse)

Bioinformatics

• Gene Symbol: HCAR3
• Entrez:
  • 8843(Human)
• Uniprot:
  • P49019(Human)