GPER/GPR30 Antibody (730737) [Unconjugated]

GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human GPER Monoclonal Antibody (Catalog # MAB5534) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 730737
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GPER/GPR30 Antibody (730737) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GPER
  Met1-Ser62
  Accession # Q99527
• Specificity: Detects human GPER in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPER
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPER/GPR30 Antibody (730737) [Unconjugated]

• CEPR
• CEPRG-protein coupled receptor 30
• Chemoattractant receptor-like 2
• chemokine receptor-like 2
• CMKRL2
• CMKRL2MGC99678
• constitutively expressed peptide-like receptor
• DRY12
• DRY12IL8-related receptor DRY12
• FEG-1
• FEG-1mER
• Flow-induced endothelial G-protein coupled receptor 1
• G protein-coupled estrogen receptor 1
• G protein-coupled receptor 30
• GPCR-BR
• GPER
• GPR30
• GPR30GPCR-Br
• G-protein coupled estrogen receptor 1
• heptahelix receptor
• LERGU
• LERGU2
• leucine rich protein in GPR30 3'UTR
• LyGPR
• Lymphocyte-derived G-protein coupled receptor
• Membrane estrogen receptor

Background

GPER (G-Protein Coupled Estrogen Receptor 1; also GPR30, DRY12 and mER) is a 44 kDa, seven transmembrane (TM) member of the GPR-1 family of molecules. It is expressed on/in neurons, monocytes and endothelial cells. Its exact location is unclear; it has been described in both the cell membrane and ER. Human GPER is 375 amino acids (aa) in length. It contains an N-terminal extracellular region (aa 1-62), a series of seven TM domains (aa 63-327), and a C-terminal cytoplasmic tail (aa 328-375). The initial function attributed to GPER was that of a membrane receptor for estrogen. There are two potential splice variants for GPER. One shows a deletion of aa 32-49, while a second shows a 99 aa substitution for aa 308-375. Over aa 1-62, human GPER shares 57% aa identity with mouse GPER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPER/GPR30 Antibody (MAB5534)(1)

Publication using MAB5534

• Chatterjee S, Kang SD, Alam S et al. Tissue-specific gene expression during productive HPV16 infection of cervical, foreskin and tonsil epithelium J. Virol. 2019-06-12 [PMID: 31189705]

Bioinformatics

• Gene Symbol: GPER
• Uniprot:
  • Q99527()