gp130/CD130 Antibody (29104) [Unconjugated]

Recombinant Human gp130 protein was serially diluted 2-fold and captured by Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB2281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 29104
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

gp130/CD130 Antibody (29104) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
  Leu24-Glu619 (Glu619Asp)
  Accession # P40189
• Specificity: Detects human gp130 in direct ELISAs. In direct ELISAs no cross‑reactivity with recombinant mouse gp130 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6ST
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gp130/CD130 Antibody (29104) [Unconjugated]

• CD130 antigen
• CD130
• CDw130
• DKFZp564F053
• gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• gp130
• IL-6 receptor subunit beta
• IL-6R subunit beta
• IL-6RB
• IL-6R-beta
• IL6ST
• interleukin 6 signal transducer (gp130, oncostatin M receptor)
• interleukin receptor beta chain
• interleukin-6 receptor subunit beta
• Interleukin-6 signal transducer
• Membrane glycoprotein 130
• membrane glycoprotein gp130
• Oncostatin-M receptor subunit alpha

Background

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

1. Wang, Y. et al. (1992) Genomics 14:666.
2. Muller-Newen, G. (2003) Sci. STKE pe40.
3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gp130/CD130 Antibody (MAB2281)(6)

Publications using MAB2281

• Karavyraki M, Porter RK. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma Medical Oncology 4/29/2022 [PMID: 35484352]
• Karavyraki M, Porter RK. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma Medical Oncology 2022-04-29 [PMID: 35484352]
• H Wang, W Hou, A Perera, C Bettler, JR Beach, X Ding, J Li, MF Denning, A Dhanarajan, SJ Cotler, C Joyce, J Yin, F Ahmed, LR Roberts, W Qiu Targeting EphA2 suppresses hepatocellular carcinoma initiation and progression by dual inhibition of JAK1/STAT3 and AKT signaling Cell Reports, 2021-02-23;34(8):108765. 2021-02-23 [PMID: 33626345] (Western Blot, Human)
• Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells Cell. Physiol. Biochem., 2017-10-05;43(3):1198-1206. 2017-10-05 [PMID: 28977795] (Western Blot, Human)
• Campbell I, Erta M, Lim S, Frausto R, May U, Rose-John S, Scheller J, Hidalgo J Trans-signaling is a dominant mechanism for the pathogenic actions of interleukin-6 in the brain. J Neurosci, 2014-02-12;34(7):2503-13. 2014-02-12 [PMID: 24523541] (Western Blot, Mouse)
• Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-25;292(1):L199-206. 2006-08-25 [PMID: 16936245] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL6ST
• Uniprot:
  • P40189()