gp130/CD130 Antibody (125605) [Unconjugated]

Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the 7TD1mouse hybridoma line. (orange line). Proliferation elicited by Recombinant Human IL-6 (25 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4682). The ND50 is typically 0.15-1 µg/mL.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: 125605
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

gp130/CD130 Antibody (125605) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse gp130
  Gln23-Glu617
  Accession # Q00560
• Specificity: Detects mouse gp130 ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human gp130, recombinant mouse (rm) rmIL-6, or rmIL-6R is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL6ST
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization
• Application Notes: ELISA Detection: Mouse gp130 Biotinylated Antibody (Catalog number BAF468)
  Standard: Recombinant Mouse gp130 Fc Chimera (Catalog number 468-MG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gp130/CD130 Antibody (125605) [Unconjugated]

• CD130 antigen
• CD130
• CDw130
• DKFZp564F053
• gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• gp130
• IL-6 receptor subunit beta
• IL-6R subunit beta
• IL-6RB
• IL-6R-beta
• IL6ST
• interleukin 6 signal transducer (gp130, oncostatin M receptor)
• interleukin receptor beta chain
• interleukin-6 receptor subunit beta
• Interleukin-6 signal transducer
• Membrane glycoprotein 130
• membrane glycoprotein gp130
• Oncostatin-M receptor subunit alpha

Background

gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

1. Narazaki, M. et al. (1993) Blood 82:1120.
2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gp130/CD130 Antibody (MAB4682)(5)

Publications using MAB4682

• A Weinstock, K Rahman, O Yaacov, H Nishi, P Menon, CA Nikain, ML Garabedian, S Pena, N Akbar, BE Sansbury, SP Heffron, J Liu, G Marecki, D Fernandez, EJ Brown, KV Ruggles, SA Ramsey, C Giannarell, M Spite, RP Choudhury, P Loke, EA Fisher Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis Elife, 2021-03-15;10(0):. 2021-03-15 [PMID: 33720008] (Neutralization, Mouse)
• Tessa J. Barrett, Martin Schlegel, Felix Zhou, Mike Gorenchtein, Jennifer Bolstorff, Kathryn J. Moore et al. Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis Science Translational Medicine 11/6/2019 [PMID: 31694925]
• A Dixit, J Bottek, AL Beerlage, J Schuettpel, S Thiebes, A Brenzel, C Garbers, S Rose-John, HW Mittrücker, A Squire, DR Engel Frontline Science: Proliferation of Ly6C(+) monocytes during urinary tract infections is regulated by IL-6 trans-signaling J. Leukoc. Biol., 2017-12-28;0(0):. 2017-12-28 [PMID: 28882904] (Neutralization, Mouse)
• X Wang, EB Miller, M Goswami, P Zhang, KE Ronning, SJ Karlen, RJ Zawadzki, EN Pugh, ME Burns Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130 J Neuroinflammation, 2017-06-23;14(1):121. 2017-06-23 [PMID: 28645275] (Neutralization, Mouse)
• Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS. J. Biol. Chem., 2011-07-22;286(39):34271-85. 2011-07-22 [PMID: 21784845] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL6ST
• Uniprot:
  • Q00560()