GOLPH4 Antibody (5E11) - Azide and BSA Free

Western Blot: GOLPH4 Antibody (5E11) [H00027333-M01] - GOLPH4 monoclonal antibody (M01), clone 5E11 Analysis of GOLPH4 expression in HeLa.

Immunocytochemistry/Immunofluorescence: GOLPH4 Antibody (5E11) [H00027333-M01] - Analysis of monoclonal antibody to GOLPH4 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: GOLPH4 Antibody (5E11) [H00027333-M01] - Detection limit for recombinant GST tagged GOLPH4 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GOLPH4 Antibody (5E11) - Azide and BSA Free Summary

• Immunogen: GOLPH4 (NP_055311.1, 473 a.a. ~ 568 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HQEQLRQQAHYDAMDNDIVQGAEDQGIQGEEGAYERDNQHQDEAEGDPGNRHEPREQGPREADPESEADRAAVEDINPADDPNNQGEDEFEEAEQV
• Marker: Golgi Apparatus Marker
• Specificity: GOLPH4 (5E11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLIM4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GOLPH4 Antibody (5E11) - Azide and BSA Free

• GIMPc
• GIMPCcis
• golgi integral membrane protein 4
• Golgi phosphoprotein 4130 kDa golgi-localized phosphoprotein
• Golgi phosphoprotein of 130 kDa
• GPP130Golgi-localized phosphoprotein of 130 kDa

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLIM4
• Entrez:
  • 27333(Human)
• OMIM:
  • 606805(Human)
• Uniprot:
  • NP_055313(Human)