GNG11 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

GNG11 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human GNG11.
  
  

  Source: E. coli

  Amino Acid Sequence: EQLRKEVKLQRQQVSKCSEEIKNY

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: GNG11
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-81811.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for GNG11 Recombinant Protein Antigen

• GNGT11G protein gamma-11 subunit
• guanine nucleotide binding protein (G protein), gamma 11
• guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-11 subunit
• guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11

Background

GNG11, also known as Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11, is a 73 amino acid that is 8 kDa, plays a role in transmembrane signaling system, its beta and gamma chains are essential for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. This protein has been linked to splenic marginal zone lymphoma disease. The GNG11 protein has shown an interaction with ZHX1, GNB1, SMURF1, AMOTL2, GCG, and over 20 other proteins in several pathways including chemokine signaling pathway, glutamatergic synapse, cholinergic synapse, signal transduction cAMP signaling, G-protein signaling regulation of p38 and JNK signaling mediated by G-proteins, G-protein signaling G-Protein alpha-i signaling cascades, inhibitory action of lipoxins and resolvin E1 on neutrophil functions, signal transduction activation of PKC via G-Protein coupled receptor, molecular mechanisms of cancer, intracellular calcium signaling, CDK5 pathway, JNK pathway, and ERK5 signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GNG11