Human GM-CSFR alpha ELISA Kit (Colorimetric) Summary
| Description |
Incubation: 3 hours 50 minutes |
| Specificity |
Recognizes both natural and recombinant human soluble GM-CSFR alpha. No cross reactivity with other human soluble receptors. |
| Standard Curve Range |
31.25-1000 pg/ml |
| Sensitivity |
16 pg/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 4 |
| Intra-Assay |
CV% < 3.4 |
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CSF2RA |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Biotinylated secondary antibody
- Buffers
- Controls (where applicable)
- Detailed procedure.
- Pre-coated strip plates
- Standards
- Stop reagents
- Streptavidin-HRP
- TMB
|
Alternate Names for Human GM-CSFR alpha ELISA Kit (Colorimetric)
Background
GM-CSF Receptor alpha is encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for GM-CSFR alpha ELISA Kit (NBP3-14630). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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