Glypican 4 Antibody (965523) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA
• Clone: 965523
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glypican 4 Antibody (965523) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Glypican 4
  Met1-Ser529
  Accession # O75487
• Specificity: Detects human Glypican 4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 4 Antibody (965523) [Unconjugated]

• Glypican 4
• glypican proteoglycan 4
• glypican-4
• GPC4
• K-glypican
• K-glypicandJ900E8.1 (glypican 4)

Background

Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).

1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
3. Ussar, S. et al. (2012) Diabetes 61:2289.
4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
8. Strate, I. et al. (2015) Development 142:1767.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75487()