Glyoxalase II/HAGH Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC201109 and based on accession number NM_005326. The protein contains a C-terminal DDK tag.
Homo sapiens hydroxyacylglutathione hydrolase (HAGH), transcript variant 1, mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for Glyoxalase II/HAGH
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Glyoxalase II/HAGH Overexpression Lysate (Native)
- EC 126.96.36.199
- GLO2hydroxyacyl glutathione hydrolase
- Glx II
- Glyoxalase II
- hydroxyacylglutathione hydrolase
- hydroxyacylglutathione hydrolase, mitochondrial
- hydroxyacylglutathione hydroxylase
The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the mitochondrially localized isoform (1). Translation may also initiate at a downstream in-frame ATG to encode the cytosolic form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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