Human Glyoxalase II/HAGH ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Human Glyoxalase II/HAGH, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Hu
• Applications: ELISA
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Glyoxalase II/HAGH ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HAGH

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Glyoxalase II/HAGH ELISA Kit (Colorimetric)

• EC 3.1.2.6
• GLO2hydroxyacyl glutathione hydrolase
• Glx II
• GLX2
• GLXII
• Glyoxalase II
• HAGH
• HAGH1GLX2
• hydroxyacylglutathione hydrolase
• hydroxyacylglutathione hydrolase, mitochondrial
• hydroxyacylglutathione hydroxylase

Background

HAGH, also known as Hydroxyacylglutathione hydrolase mitochondrial, has a 308 amino acid long isoform that is 34 kDa and located in the mitochondrion matrix and a short 260 amino acid isoform that is 29 kDa and located in the cytoplasm; is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Studies are being performed on the relationship of this protein to glyoxalase ii deficiency, breast carcinoma, carcinoma, familial Mediterranean fever, muscular dystrophy, thrombocytosis, neisseria meningitides, hyperglycemia, bladder carcinoma, alzheimer's disease, prostate cancer, hepatitis b, and prostatitis. HAGH protein involvement has been observed with relation to pyruvate metabolism, secondary metabolite metabolism, methylglyoxal degradation, and (R)-lactate from methylglyoxal pathways where it interacts with MYOC, PRDX2, VPS72, SOD1, and DR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glyoxalase II/HAGH ELISA Kit (NBP3-49769). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: HAGH