Recombinant Human Glyoxalase II/HAGH Protein

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SDS-Page: Recombinant Human Glyoxalase II/HAGH Protein [NBP1-50892] - 15% SDS-Page (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Glyoxalase II/HAGH Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-260 of Human Glyoxalase II/HAGH

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD

Details of Functionality
Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
HAGH
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl Buffer (pH 8.5) containing 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Glyoxalase II/HAGH Protein

  • EC 3.1.2.6
  • GLO2hydroxyacyl glutathione hydrolase
  • Glx II
  • GLX2
  • GLXII
  • Glyoxalase II
  • HAGH
  • HAGH1GLX2
  • hydroxyacylglutathione hydrolase
  • hydroxyacylglutathione hydrolase, mitochondrial
  • hydroxyacylglutathione hydroxylase

Background

HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol HAGH
Entrez