Glyoxalase II/HAGH Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Glyoxalase II/HAGH Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Glyoxalase II
  Met49-Ile254
  Accession # Q16775
• Specificity: Detects human, mouse, and rat Glyoxalase II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Glyoxalase I is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glyoxalase II/HAGH Antibody [Alexa Fluor® 647]

• EC 3.1.2.6
• GLO2hydroxyacyl glutathione hydrolase
• Glx II
• GLX2
• GLXII
• Glyoxalase II
• HAGH
• HAGH1GLX2
• hydroxyacylglutathione hydrolase
• hydroxyacylglutathione hydrolase, mitochondrial
• hydroxyacylglutathione hydroxylase

Background

Glyoxalase II (GLO2; also hydroxyacylglutathione hydrolase 2 and Glx II) is a monomeric, cytosolic 29 kDa member of the Glyoxylase II family, metallo-beta -lactamase superfamily of enzymes. It is expressed in liver and kidney, and converts GLO1-generated lactoylglutathione into lactate and GSH. It is up‑regulated by p63 and p73, and thus serves as a p53-associated survival factor. Human GLO2 is 260 amino acids (aa) in length. It binds two atoms of Zn and contains one substrate binding region (aa 143‑145 plus 249‑252). The 29 kDa form represents 80‑90% of cellular GLO2. There is also a 32‑33 kDa, 308 aa mitochondrial isoform that arises via the use of an alternative start site. Its function is unclear, given that mitochondria do not contain GLO1. There is an additional potential isoform that shows an insertion of nine aa after Thr167. Human GLO2 shares approximately 90% aa identity with mouse GLO2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.