Glyoxalase I Antibody (JU44-11)

Western Blot: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.Positive control:Lane 1: Mouse testis tissue Lane 2: C2C12Lane 3: 293 Lane 4: Rat spleen tissue

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Staining GLO1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Analysis of paraffin-embedded rat epididymis tissue using anti-GLO1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Analysis of Hela cells with GLO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Staining GLO1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Staining GLO1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (JU44-11) [NBP2-75514] - Analysis of paraffin-embedded human thyroid gland tissue using anti-GLO1 antibody. Counter stained with hematoxylin.

Product Discontinued

Glyoxalase I Antibody (JU44-11) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Glyoxalase I aa 1-120 / 184. (SwissProt: Q04760 Human; SwissProt: SwissProt: Q9CPU0 Mouse; SwissProt: SwissProt: Q6P7Q4 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GLO1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glyoxalase I Antibody (JU44-11)

• Aldoketomutase
• EC 4.4.1.5
• GLO1
• GLOD1
• Glx I
• GLYI
• glyoxalase domain containing 1
• Glyoxalase I
• glyoxalase Ialdoketomutase
• Ketone-aldehyde mutase
• lactoyl glutathione lyase
• lactoylglutathione lyase
• Methylglyoxalase
• S-D-lactoylglutathione methylglyoxal lyase

Background

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLO1