Glyoxalase I Antibody (4C12)

Sandwich ELISA: Glyoxalase I Antibody (4C12) [H00002739-M01] - Detection limit for recombinant GST tagged GLO1 is 1 ng/ml as a capture antibody.

Product Discontinued

Glyoxalase I Antibody (4C12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GLO1 (AAH11365.1, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEPQPPSGGLTDEAALSYCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKAT
• Specificity: GLO1 - glyoxalase I (4C12)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLO1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot 1:100-1:2000
• Application Notes: It has been used for ELISA. Use in Western blot and Immunohistochemistry reported in scientific literature (PMID: 20093988).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glyoxalase I Antibody (4C12)

• Aldoketomutase
• EC 4.4.1.5
• GLO1
• GLOD1
• Glx I
• GLYI
• glyoxalase domain containing 1
• Glyoxalase I
• glyoxalase Ialdoketomutase
• Ketone-aldehyde mutase
• lactoyl glutathione lyase
• lactoylglutathione lyase
• Methylglyoxalase
• S-D-lactoylglutathione methylglyoxal lyase

Background

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glyoxalase I Antibody (H00002739-M01)(2)

Publications using H00002739-M01

• Havas F, Krispin S, Cohen M et al. A Dunaliella salina Extract Counteracts Skin Aging under Intense Solar Irradiation Thanks to Its Antiglycation and Anti-Inflammatory Properties Marine drugs 2022-01-27 [PMID: 35200634] (IF/IHC, Human)
• Bair WB 3rd, Cabello CM, Uchida K et al. GLO1 overexpression in human malignant melanoma. Melanoma Res. 2010-04-01 [PMID: 20093988] (IF/IHC, WB, Human)
  
  Details:
  IHC (paraffin) & labelled with a GLO1 antibody (Fig 2): 1. Malignant Melanoma: IMH-3692. Skin Cancer & Normal Skin: IMH-323.

Bioinformatics

• Gene Symbol: GLO1
• Entrez:
  • 2739(Human)
• Uniprot:
  • AAH11365(Human)