Glycoprotein V/CD42d Antibody [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Glycoprotein V/CD42d Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Glycoprotein V/CD42d
  Gln17-Gly522
  Accession # NP_032174
• Specificity: Detects mouse Glycoprotein V/CD42d in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glycoprotein V/CD42d Antibody [Alexa Fluor® 405]

• platelet glycoprotein V
• CD42d
• glycoprotein V (platelet)
• Glycoprotein V
• GP5
• GPV

Background

GP-V (platelet glycoprotein V; also CD42d) is a 78-88 kDa member of the leucine-rich (LR) repeat family of proteins. It is expressed on the surface of platelets where it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for both thrombin and von Willebrand factor. In addition, GP-V is normally a receptor for collagen. Following receptor complex activation due to thrombin cleavage of GP-V, the collagen-binding activity of GP-V is lost, limiting thrombus growth at sites of clot formation. Mature mouse GP-V is a 551 amino acid (aa) type I transmembrane glycoprotein. In its 506 aa extracellular domain (aa 17-522), GP-V contains 16 LR repeats (aa 17-474), and terminates with a short 24 aa cytoplasmic tail. Based on human, mouse GP-V might be naturally cleaved by thrombin and ADAM17, generating soluble fragments that range from 70-82 kDa in size. Over aa 17-522, mouse GP-V shares 83% and 70% aa sequence identity with rat and human GP-V, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.