Glycoprotein V/CD42d Antibody (508209) [Unconjugated]

Human blood‑derived CD41+ platelets were stained with Mouse Anti-Human Glycoprotein V/CD42d Mono­clonal Antibody (Catalog # MAB4249, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 508209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Glycoprotein V/CD42d Antibody (508209) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
  Gln17-Gly523
  Accession # P40197
• Specificity: Detects human Glycoprotein V/CD42d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GP5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glycoprotein V/CD42d Antibody (508209) [Unconjugated]

• platelet glycoprotein V
• CD42d
• glycoprotein V (platelet)
• Glycoprotein V
• GP5
• GPV

Background

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
6. Wolff, V. et al. (2005) Stroke 36:e17.
7. Javela, K. et al. (2005) Transfusion 45:1504.
8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
9. Kahn, M.L. (1999) Blood 94:4112.
10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
12. Moog, S. et al. (2001) Blood 98:1038.
13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
14. Ni, H. et al. (2001) Blood 98:368.
15. Andrews, R.K. et al. (2001) Blood 98:681.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GP5
• Uniprot:
  • P40197(Human)