Glycoprotein V/CD42d Antibody (508209) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: Flow
• Clone: 508209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Glycoprotein V/CD42d Antibody (508209) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
  Gln17-Gly523
  Accession # P40197
• Specificity: Detects human Glycoprotein V/CD42d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GP5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glycoprotein V/CD42d Antibody (508209) [Alexa Fluor® 594]

• platelet glycoprotein V
• CD42d
• glycoprotein V (platelet)
• Glycoprotein V
• GP5
• GPV

Background

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
6. Wolff, V. et al. (2005) Stroke 36:e17.
7. Javela, K. et al. (2005) Transfusion 45:1504.
8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
9. Kahn, M.L. (1999) Blood 94:4112.
10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
12. Moog, S. et al. (2001) Blood 98:1038.
13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
14. Ni, H. et al. (2001) Blood 98:368.
15. Andrews, R.K. et al. (2001) Blood 98:681.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GP5
• Uniprot:
  • P40197(Human)