Glycoprotein V/CD42d Antibody (508209) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
508209
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Glycoprotein V/CD42d Antibody (508209) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
Gln17-Gly523
Accession # P40197
Specificity
Detects human Glycoprotein V/CD42d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GP5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glycoprotein V/CD42d Antibody (508209) [Alexa Fluor® 594]

  • platelet glycoprotein V
  • CD42d
  • glycoprotein V (platelet)
  • Glycoprotein V
  • GP5
  • GPV

Background

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

  1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
  2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
  3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
  4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
  5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
  6. Wolff, V. et al. (2005) Stroke 36:e17.
  7. Javela, K. et al. (2005) Transfusion 45:1504.
  8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
  9. Kahn, M.L. (1999) Blood 94:4112.
  10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
  11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
  12. Moog, S. et al. (2001) Blood 98:1038.
  13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
  14. Ni, H. et al. (2001) Blood 98:368.
  15. Andrews, R.K. et al. (2001) Blood 98:681.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB4249U
Alexa Fluor 405 FAB4249V
Alexa Fluor 488 FAB4249G
Alexa Fluor 594 FAB4249T
Alexa Fluor 647 FAB4249R
Alexa Fluor 700 FAB4249N
Alexa Fluor 750 FAB4249S
Allophycocyanin FAB4249A
Phycoerythrin FAB4249P

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Bioinformatics

Gene Symbol GP5
Uniprot