Glycogen Phosphorylase BB/GPBB Antibody

Images

 
Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:2000.
Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Dilution: 1:500.
Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - detects PYGB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Dilution: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Mk, ShSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.84 mg/ml

Order Details

Glycogen Phosphorylase BB/GPBB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GPBB. The exact sequence is proprietary.
Predicted Species
Rat (91%), Sheep (92%), Monkey (98%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYGB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (85%), Chicken (86%), Rhesus Monkey (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.84 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glycogen Phosphorylase BB/GPBB Antibody

  • Brain glycogen phosphorylase
  • EC 2.4.1.1
  • Glycogen phosphorylase B
  • Glycogen phosphorylase brain form
  • Glycogen Phosphorylase Isoenzyme BB
  • GPBB
  • MGC9213
  • Phosphorylase glycogen brain
  • PYGB

Background

This glycogen phosphorylase is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PYGB
Entrez
Uniprot