Glycogen Phosphorylase BB/GPBB Antibody

Images

 
Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPBB antibody diluted at 1:1000. The ...read more
Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Dilution: 1:500.
Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - detects PYGB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Dilution: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, ShSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glycogen Phosphorylase BB/GPBB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GPBB. The exact sequence is proprietary.
Predicted Species
Sheep (92%), Rhesus Macaque (98%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYGB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32799 in the following applications:

  • WB
    6 publications
  • wb
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30954669). Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%), Chicken (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Glycogen Phosphorylase BB/GPBB Antibody

  • Brain glycogen phosphorylase
  • EC 2.4.1.1
  • Glycogen phosphorylase B
  • Glycogen phosphorylase brain form
  • Glycogen Phosphorylase Isoenzyme BB
  • GPBB
  • MGC9213
  • Phosphorylase glycogen brain
  • PYGB

Background

This glycogen phosphorylase is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Glycogen Phosphorylase BB/GPBB Antibody (NBP1-32799)(7)

We have publications tested in 2 confirmed species: Rat, human.

We have publications tested in 2 applications: WB, wb.


Filter By Application
WB
(6)
wb
(1)
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Rat
(6)
human
(1)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-32799 Applications Species
Uddin MM, Ibrahim MMH, Briski KP Sex-dimorphic neuroestradiol regulation of ventromedial hypothalamic nucleus glucoregulatory transmitter and glycogen metabolism enzyme protein expression in the rat BMC Neurosci Nov 25 2020 [PMID: 33238883] (wb, human) wb human
Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP Sex differences in glucoprivic regulation of glycogen metabolism in hypothalamic primary astrocyte cultures: Role of estrogen receptor signaling Mol. Cell. Endocrinol. Aug 24 2020 [PMID: 32853745] (WB, Rat) WB Rat
Mahmood A S M H, Napit P R et al. Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression. ASN Neuro Mar 4 2020 [PMID: 32233668] (WB, Rat) WB Rat
Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP Sex-specific estrogen regulation of hypothalamic astrocyte estrogen receptor expression and glycogen metabolism in rats Mol. Cell. Endocrinol. Jan 11 2020 [PMID: 31931041] (WB, Rat) WB Rat
Briski K, Mandal S Hindbrain lactoprivic regulation of hypothalamic neuron transactivation and gluco-regulatory neurotransmitter expression: Impact of antecedent insulin-induced hypoglycemia Neuropeptides Aug 1 2019 [PMID: 31488323] (WB, Rat) WB Rat
Anczurowski M, Sugata K, Matsunaga Y et al. Hindbrain Estrogen Receptor Regulation of Ventromedial Hypothalamic Glycogen Metabolism and Glucoregulatory Transmitter Expression in the Hypoglycemic Female Rat Neuroscience May 12 2019 [PMID: 31085279] (WB, Rat) WB Rat
Ali, MH;Napit, PR;Mahmood, ASMH;Bheemanapally, K;Alhamami, HN;Uddin, MM;Mandal, SK;Ibrahim, MMH;Briski, KP; Hindbrain estrogen receptor regulation of ventromedial hypothalamic glycogen metabolism and glucoregulatory transmitter expression in the hypoglycemic male rat Neuroscience Apr 5 2019 [PMID: 30954669] (WB, Rat) WB Rat

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Bioinformatics

Gene Symbol PYGB
Entrez
Uniprot